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Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.
Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.
Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SHANK3 Antibody

CATALOG NUMBER: 6835

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.
The immunogen is located within amino acids 890 - 940 of SHANK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SHANK3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SHANK3
Additional Names:
SHANK3 Antibody: PSAP2, SCZD15, PROSAP2, SPANK-2, DEL22q13.3, KIAA1650, PSAP2, SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2, Shank3
Protein Accession Number:
NP_001073889
Protein Gi Number:
122937241
Ncbi Gene Id Number:
85358
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SHANK3 Antibody: SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.
Background Reference 1:
Sheng M and Kim E. The Shank family of scaffold proteins. J. Cell Sci. 2000; 113:1851-6.
Background Reference 2:
Park E, Na M, Choi J. The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the PIX guanine nucleotide exchange factor for Rac1 and Cdc42. J. Biol. Chem. 2004; 278:19220-9
Background Reference 3:
Lim S, Naisbitt S, Yoon J, et al. Characterization of the Shank family of synaptic proteins. Multiple genes, alternative splicing and differential expression in brain and development. J. Biol. Chem. 1999; 274:29510-8.
Background Reference 4:
Durand CM, Betancur C, Boeckers TM, et al. Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are associated with autism spectrum disorders. Nat. Genet. 2007; 39: 25-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6835

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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