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Western blot analysis of extracts of various cell lines, using SHC1 antibody (23-133) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using SHC1 antibody (23-133) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SHC1 antibody (23-133) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SHC1 antibody (23-133) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SHC1 antibody (23-133) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SHC1 antibody (23-133) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

SHC1 Antibody

CATALOG NUMBER: 23-133

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human SHC1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
U-251MG
Positive Control 3:
MCF7
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 46kDa, 52kDa, 66kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SHC1
Additional Names:
SHC1, FLJ26504, SHC, SHCA, p52SHC, p66, p66SHC
Ncbi Gene Id Number:
6464
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-133

List Size:
100 uL

List Price:

$436.00

Datasheet
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