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Western blot analysis in Jurkat cell line lysates (35ug/lane).
SHCBP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

SHCBP1 Antibody

CATALOG NUMBER: 61-925

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SHCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-528 amino acids from the C-terminal region of human SHCBP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SHCBP1
Additional Names:
SHC SH2 domain-binding protein 1, SHCBP1
Protein Accession Number:
Q8NEM2
Protein Gi Number:
296452958
Ncbi Gene Id Number:
79801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 2:
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Background Reference 3:
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Background Reference 4:
Obuse, C., et al. Genes Cells 9(2):105-120(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-925

List Size:
400 ul

List Price:

$495.00

Datasheet
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