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Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 μg/ml.
Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 μg/ml.
Western blot analysis of SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SHISA3 Antibody

CATALOG NUMBER: 7275

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SHISA3 antibody was raised against a 15 amino acid peptide near the center of human SHISA3 .
The immunogen is located within amino acids 120 - 170 of SHISA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SHISA3 Antibody can be used for detection of SHISA3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SHISA3 antibody is predicted to not cross-react with other SHISA protein family members.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 28 kDa
Purification:
SHISA3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SHISA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SHISA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SHISA3
Additional Names:
SHISA3 Antibody: hShisa3, Protein shisa-3 homolog
Protein Accession Number:
NP_001073974
Protein Gi Number:
122937450
Ncbi Gene Id Number:
152573
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SHISA3 Antibody: SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
Background Reference 1:
Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92.
Background Reference 2:
Pei J and Grishin NV. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell Signal. 2012; 24:758-69.
Background Reference 3:
Yamamoto A, Nagano T, Takehara S, et al. Shisa promotes head formation through the inhibition of receptor protein maturation for the caudalizing factors, Wnt and FGF. Cell 2005; 120:223-35

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7275

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

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