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Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SHISA4 in mouse brain tissue with SHISA4 antibody at 2.5 μg/ml.
Immunofluorescence of SHISA4 in mouse brain tissue with SHISA4 antibody at 20 μg/ml.
Western blot analysis of SHISA4 in human brain tissue lysate with SHISA4 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SHISA4 Antibody

CATALOG NUMBER: 7277

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4 .
The immunogen is located within amino acids 100 - 150 of SHISA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SHISA4 antibody is predicted to not cross-react with other SHISA protein family members.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 24 kDa
Purification:
SHISA4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SHISA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SHISA4
Additional Names:
SHISA4 Antibody: TMEM58, C1orf40, TMEM58, UNQ583/PRO1153, Protein shisa-4, Transmembrane protein 58
Protein Accession Number:
NP_937792
Protein Gi Number:
269973884
Ncbi Gene Id Number:
149345
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SHISA4 Antibody: SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
Background Reference 1:
Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92.
Background Reference 2:
Pei J and Grishin NV. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell Signal. 2012; 24:758-69.
Background Reference 3:
Yamamoto A, Nagano T, Takehara S, et al. Shisa promotes head formation through the inhibition of receptor protein maturation for the caudalizing factors, Wnt and FGF. Cell 2005; 120:223-35.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7277

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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