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Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).
SHMT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.

SHMT1 Antibody

CATALOG NUMBER: 57-723

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SHMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human SHMT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SHMT1
Additional Names:
Serine hydroxymethyltransferase, cytosolic, SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT1
Protein Accession Number:
P34896
Protein Gi Number:
462184
Ncbi Gene Id Number:
6470
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the cellular form of serinehydroxymethyltransferase, a pyridoxal phosphate-containing enzymethat catalyzes the reversible conversion of serine andtetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate.This reaction provides one carbon units for synthesis ofmethionine, thymidylate, and purines in the cytoplasm. This gene islocated within the Smith-Magenis syndrome region on chromosome 17.Alternative splicing of this gene results in 2 transcript variantsencoding 2 different isoforms. Additional transcript variants havebeen described, but their biological validity has not beendetermined.
Background Reference 1:
Porter, K.E., et al. Environ. Res. 110(6):580-587(2010)
Background Reference 2:
Summers, C.M., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(8):679-688(2010)
Background Reference 3:
Vijayakrishnan, J., et al. Haematologica 95(8):1405-1414(2010)
Background Reference 4:
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-723

List Size:
400 ul

List Price:

$495.00

Datasheet
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