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Western blot analysis of extracts of various cell lines, using SHMT2 antibody (14-280) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody (14-280) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SHMT2 antibody (14-280). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SHMT2 antibody (14-280) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.

SHMT2 Antibody

CATALOG NUMBER: 14-280

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
OVCAR3
Positive Control 3:
22Rv1
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse kidney
Predicted Molecular Weight:
Observed: 52kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SHMT2
Additional Names:
SHMT2, GLYA, SHMT, HEL-S-51e
Ncbi Gene Id Number:
6472
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-280

List Size:
100 uL

List Price:

$540.00

Datasheet
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