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45-128 (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-128 (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SHP-1 Antibody

CATALOG NUMBER: 45-128

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HTKNKREEKVKKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and U937 (calculated MW of 67.7kDa according to NP_536858.1 and 67.6kDa according to NP_002822.2). Recommended concentration: 0.2-0.5ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2)
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTPN6
Additional Names:
PTPN6, SHP1 , protein tyrosine phosphatase, non-receptor type 6 , HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, 70 kda SHP-1L protein, hematopoietic cell phosphatase, hematopoietic cell protein-tyrosine phosphatase, protein-tyrosine phosphatase 1C
Protein Accession Number:
NP_536858.1,NP_002822.2
Protein Gi Number:
18104991
Ncbi Gene Id Number:
5777
Background Reference 1:
Dubois MJ, Bergeron S, Kim HJ, Dombrowski L, Perreault M, Fournes B, Faure R, Olivier M, Beauchemin N, Shulman GI, Siminovitch KA, Kim JK, Marette A. The SHP-1 protein tyrosine phosphatase negatively modulates glucose homeostasis. Nat Med. 2006 May;12(5):549-56. Epub 2006 Apr 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-128

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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