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Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SHROOM1 Antibody

CATALOG NUMBER: 7113

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal SHROOM1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SHROOM1.
The immunogen is located within the last 50 amino acids of SHROOM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
94 kDa
Purification:
SHROOM1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SHROOM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SHROOM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SHROOM1
Additional Names:
SHROOM1 Antibody: APXL2, APXL2, KIAA1960, Protein Shroom1, Apical protein 2
Protein Accession Number:
NP_001166171
Protein Gi Number:
289577098
Ncbi Gene Id Number:
134549
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SHROOM1 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.
Background Reference 1:
Lee C, Le MP, and Wallingford JB. The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets. Dev. Dyn. 2009; 238:1480-91.
Background Reference 2:
Hildebrand JD and Soriano P. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell 1999; 99:486-97
Background Reference 3:
Hildebrand JD. Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network. J. Cell Sci. 2005; 118:5191-203.
Background Reference 4:
Sevilla-Perez J, Konigshoff M, Kwapiszewska G, et al. Shroom expression is attenuated in pulmonary arterial hypertension. Eur. Respir. J. 2008; 32:871-880.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7113

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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