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Antibody used in WB on Human Jurkat 1.25 ug/ml.
Antibody used in WB on Human HEK293T cells at: 1:1000.
Antibody used in WB on Human Jurkat 1.25 ug/ml.

SIAH1 Antibody

CATALOG NUMBER: 28-017

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIAH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIAH1 antibody can be used for detection of SIAH1 by ELISA at 1:312500. SIAH1 antibody can be used for detection of SIAH1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
35 kDa, 31 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SIAH1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SIAH1
Additional Names:
SIAH1, SIAH1A
Protein Accession Number:
NP_001006611
Protein Gi Number:
55749557
Ncbi Gene Id Number:
6477
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Background Reference 1:
Avraham, E., et al., (2005) J. Biol. Chem. 280 (52), 42877-42886.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-017

List Size:
100 ul

List Price:

$473.00

Datasheet
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