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45-144 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-144 (1ug/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-144 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

SIAH1 Antibody

CATALOG NUMBER: 45-144

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: SRQTATALPTGTSKC
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Liver lysate and in Mouse and Rat Brain and Liver lysates (calculated MW of 31.1kDa according to NP_003022.3). Recommended concentration: 1-3ug/ml. Primary incubated for 1 hour.
Specificity:
This product is expected to recognize both reported isoforms (NP_003022.3; NP_001006611.1)
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SIAH1
Additional Names:
SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog
Protein Accession Number:
NP_003022.3,NP_001006611.1
Protein Gi Number:
63148618
Ncbi Gene Id Number:
6477
Background Reference 1:
Susini L, Passer BJ, Amzallag-Elbaz N, Juven-Gershon T, Prieur S, Privat N, Tuynder M, Gendron MC, Israel A, Amson R, Oren M, Telerman A. Siah-1 binds and regulates the function of Numb. Proc Natl Acad Sci USA. 2001 Dec 18;98(26):15067-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-144

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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