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Western blot analysis of Siglec11 in HepG2 cell lysate with Siglec11 antibody at 1 μg/mL.
Immunohistochemistry of Siglec11 in human liver tissue with Siglec11 antibody at 5 μg/mL.
Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 μg/mL.
Western blot analysis of Siglec11 in HepG2 cell lysate with Siglec11 antibody at 1 μg/mL.
Trial Size

Siglec11 Antibody

CATALOG NUMBER: 5151

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11.
The immunogen is located within the last 50 amino acids of Siglec11.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
Siglec11 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Siglec11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Siglec11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIGLEC11
Additional Names:
Siglec11 Antibody: Sialic acid-binding Ig-like lectin 11, Sialic acid-binding lectin 11
Protein Accession Number:
NP_443116
Protein Gi Number:
205830470
Ncbi Gene Id Number:
114132
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Siglec11 Antibody: Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.
Background Reference 1:
Crocker PR and Varki A. Siglecs, sialic acids, and innate immunity. Trends Immunol.2001; 22:337-42.
Background Reference 2:
Angata T, Kerr SC, Greaves DR, et al. Cloning and characterization of human siglec-11. A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia. J. Biol. Chem.2002; 27:24466-74.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5151

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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