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Western blot analysis in HepG2 cell line lysates (35ug/lane).
SIGLEC8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

SIGLEC8 Antibody

CATALOG NUMBER: 57-823

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SIGLEC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human SIGLEC8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIGLEC8
Additional Names:
Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2, SIGLEC8, SAF2
Protein Accession Number:
Q9NYZ4
Protein Gi Number:
25009268
Ncbi Gene Id Number:
27181
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sialic acid-binding immunoglobulin (Ig)-like lectins, orSIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1transmembrane proteins each having a unique expression pattern,mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-likesubgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptortyrosine-based inhibitory motif, or ITIM (see MIM 604964), and amotif homologous to one identified in signaling lymphocyteactivation molecule (SLAM; MIM 603492) that mediates an associationwith SLAM-associated protein (SAP; MIM 300490) (summarized byFoussias et al., 2000 [PubMed 11095983]).
Background Reference 1:
Gao, P.S., et al. Eur. J. Hum. Genet. 18(6):713-719(2010)
Background Reference 2:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 3:
Hudson, S.A., et al. J. Pharmacol. Exp. Ther. 330(2):608-612(2009)
Background Reference 4:
Bochner, B.S. Clin. Exp. Allergy 39(3):317-324(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-823

List Size:
400 ul

List Price:

$495.00

Datasheet
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