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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Antibody is used in Western blot to detect Siglec9 in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

SIGLEC9 Antibody

CATALOG NUMBER: 58-379

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This SIGLEC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-415 amino acids from the C-terminal region of human SIGLEC9.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIGLEC9
Additional Names:
Sialic acid-binding Ig-like lectin 9, Siglec-9, CDw329, Protein FOAP-9, CD329, SIGLEC9
Protein Accession Number:
Q9Y336
Protein Gi Number:
25009270
Ncbi Gene Id Number:
27180
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon. SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Background Reference 1:
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Zhang, J.Q., et al., J. Biol. Chem. 275(29):22121-22126 (2000). Foussias, G., et al., Genomics 67(2):171-178 (2000). Angata, T., et al., J. Biol. Chem. 275(29):22127-22135 (2000). Yousef, G.M., et al., Anticancer Res. 19 (4B), 2843-2852 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-379

List Size:
400 ul

List Price:

$495.00

Datasheet
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