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Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 μg/mL.
Immunohistochemistry of SIK1 in human small intestine tissue with SIK1 antibody at 5 μg/mL.
Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 μg/mL.
Trial Size

SIK1 Antibody

CATALOG NUMBER: 6299

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (73%), Mouse: (73%)
Immunogen:
SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1.
The immunogen is located within amino acids 340 - 390 of SIK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Purification:
SIK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIK1
Additional Names:
SIK1 Antibody: MSK, SIK, SNF1LK, Salt-inducible kinase 1, SIK-1
Protein Accession Number:
NP_775490
Protein Gi Number:
116256471
Ncbi Gene Id Number:
150094
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.
Background Reference 1:
Stephenson A, Huang GY, Nguyen NT, et al. SNF1LK encodes a protein kinase that may function in cell cycle regulation. Genomics 2004; 83:1105-15
Background Reference 2:
Ruiz JC, Conlon FL, and Robertson EJ. Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech. Dev. 1994; 48:153-64
Background Reference 3:
Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J. 2004; 23:833-43.
Background Reference 4:
Sjöström M, Senstrom K, Eneling K, et al. SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Natl. Acad. Sci. USA 2007; 104:16922-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6299

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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