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Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 μg/mL.
Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SIK3 Antibody

CATALOG NUMBER: 6613

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3.
The immunogen is located within amino acids 1190 - 1240 of SIK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SIK3 antibody can be used for detection of SIK3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SIK3 are known to exist; this antibody will detect two isoforms. SIK3 antibody is predicted to not cross-react with SIK1 and SIK2.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
SIK3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SIK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIK3
Additional Names:
SIK3 Antibody: L19, QSK, SIK-3, KIAA0999, L19, Salt-inducible kinase 3
Protein Accession Number:
NP_079440
Protein Gi Number:
38569491
Ncbi Gene Id Number:
23387
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIK3 Antibody: Salt-inducible kinase family (SIKs) proteins are thought to have a role in steroidogenesis, adipogenesis or regulation of tumor malignancy. Three members (SIK1, SIK2 and SIK3) in the SIK family have been identified thus far. Their kinase domain sequences are closely homologous to those of AMP-activated protein kinase (AMPK). SIK3 can be phosphorylated by a tumor-suppressor kinase LKB1. It is highly and preferentially expressed in ovarian tumors but not in adenomyosis and leiomyoma and may be a potential diagnostic marker for ovarian cancers.
Background Reference 1:
Katoh Y, Takemori H, Horike N, et al. Salt-inducible kinase (SIK) isoforms: their involvement in steroidogenesis and adipogenesis. Mol. Cell. Endocrinol. 2004; 217:109-12.
Background Reference 2:
Al-Hakim AK, Göransson O, Deak M, et al. 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 2005; 118:5661-73.
Background Reference 3:
Charoenfuprasert S, Yang YY, Lee YC, et al. Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene 2011; 30:3570-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6613

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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