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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
SIKE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

SIKE Antibody

CATALOG NUMBER: 61-907

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This SIKE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the C-terminal region of human SIKE.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIKE1
Additional Names:
Suppressor of IKBKE 1, Suppressor of IKK-epsilon, SIKE1, SIKE
Protein Accession Number:
Q9BRV8
Protein Gi Number:
74752290
Ncbi Gene Id Number:
80143
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) andTBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) andvirus-triggered interferon activation pathways (Huang et al., 2005[PubMed 16281057]).
Background Reference 1:
Goudreault, M., et al. Mol. Cell Proteomics 8(1):157-171(2009)
Background Reference 2:
Huang, J., et al. EMBO J. 24(23):4018-4028(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-907

List Size:
400 ul

List Price:

$495.00

Datasheet
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