SILV Antibody

CATALOG NUMBER: 30-232

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SILV.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SILV antibody can be used for detection of SILV by ELISA at 1:1562500. SILV antibody can be used for detection of SILV by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
70 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SILV antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PMEL
Additional Names:
SILV, D12S53E, ME20, PMEL17, SI, SIL, gp100, P1, P100, SILV, ME20M, ME20-M
Protein Accession Number:
NP_008859
Protein Gi Number:
5902084
Ncbi Gene Id Number:
6490
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SILV could be a melanogenic enzyme. It could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, it could protect tumor cells from antibody mediated immunity.
Background Reference 1:
Theos, A.C., (2006) Dev. Cell 10 (3), 343-354.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-232

List Size:
100 ul

List Price:

$473.00

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