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Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using SIN3A antibody (16-224). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (16-224). Western blot was performed from the immunoprecipitate using SIN3A antibody (16-224) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SIN3A Antibody

CATALOG NUMBER: 16-224

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
ChIP: 1:20 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
COS7
Positive Control 3:
U-937
Positive Control 4:
MCF-7
Positive Control 5:
293T
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 165kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SIN3A
Additional Names:
SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
Ncbi Gene Id Number:
25942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-224

List Size:
100 uL

List Price:

$436.00

Datasheet
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