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Antibody used in WB on Human HCT15 at 0.2-1 ug/ml.
Antibody used in WB on Human HCT15 at 0.2-1 ug/ml.

SIN3B Antibody

CATALOG NUMBER: 26-550

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIN3B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIN3B antibody can be used for detection of SIN3B by ELISA at 1:62500. SIN3B antibody can be used for detection of SIN3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
HCT15 Cell Lysate
Predicted Molecular Weight:
133 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SIN3B antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SIN3B
Additional Names:
SIN3B, KIAA0700
Protein Accession Number:
NP_056075
Protein Gi Number:
52138513
Ncbi Gene Id Number:
23309
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIN3B acts as a transcriptional repressor. It interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also, it interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. The gene is also able to form a complex with FOXK1 which represses transcription
Background Reference 1:
Dhanda, R.S., (er) BMC Mol. Biol. 9, 8 (2008).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-550

List Size:
100 ul

List Price:

$519.00

Datasheet
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