Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.
Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.
Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.
Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.
Trial Size

SIP1 Antibody

CATALOG NUMBER: 5823

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1.
The immunogen is located within the last 50 amino acids of SIP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
SIP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GEMIN2
Additional Names:
SIP1 Antibody: SIP1, SIP1-delta, SIP1, Gem-associated protein 2, Component of gems 2, Gemin-2
Protein Accession Number:
AAB82297
Protein Gi Number:
2570925
Ncbi Gene Id Number:
8487
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIP1 Antibody: SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.
Background Reference 1:
Liu Q, Fischer U, Wang F, et al. The spinal muscle atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell1997; 90:1013-21.
Background Reference 2:
Ogawa C, Usui K, Aoki M, et al. Gemin2 plays an important role in stabilizing the survival of motor neuron complex. J. Biol. Chem.2007; 282:11122-34.
Background Reference 3:
Jablonka S, Holtmann B, Meister G, et al. Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death. Proc. Natl. Acad. Sci. USA2002; 99:10126-31.
Background Reference 4:
McWhorter ML, Boon KL, Horan ES, et al. The SMN binding protein Gemin2 is not involved in motor axon outgrowth. Dev. Neurobiol.2008; 68:182-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5823

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369