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Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 μg/mL.
Immunofluorescence of SIPA1 in human brain cells with SIPA1 antibody at 20 μg/mL.
Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 μg/mL.
Trial Size

SIPA1 Antibody

CATALOG NUMBER: 6643

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (95%)
Immunogen:
SIPA1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human SIPA1.
The immunogen is located within amino acids 40 - 90 of SIPA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SIPA1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SIPA1 are known to exist; this antibody will recognize all three isoforms. This antibody is specific for SIPA1 and will not recognize the other SIPA1 family of proteins.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
SIPA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIPA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIPA1
Additional Names:
SIPA1 Antibody: SPA1, SPA1, Signal-induced proliferation-associated protein 1, GTPase-activating protein Spa-1, Sipa-1
Protein Accession Number:
AAH10492
Protein Gi Number:
206725523
Ncbi Gene Id Number:
6494
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIPA1 Antibody: Signal-induced proliferation 1 (SIPA1) is a member of the SIPA1 family of RapGAPs, a family of mitogen-induced GTPase activating proteins. SIPA1 exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. When abnormally or prematurely expressed, SIPA1 may also hamper mitogen-induced cell cycle progression and may increase metastases in human cancers.
Background Reference 1:
Hattori M, Tsukamoto N, Nur-e-Kamal MS, et al. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol. Cell. Biol. 1995; 15:551-60.
Background Reference 2:
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci. 2009; 100:17-23.
Background Reference 3:
Kurachi H, Wada Y, Tsukamoto N, et al. Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product. J. Biol. Chem. 1997; 272:28081-8.
Background Reference 4:
Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell 2003; 4:55-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6643

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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