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Western blot analysis of SIPA1L1 in rat brain tissue lysate with SIPA1L1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 5 μg/mL.
Immunofluorescence of SIPA1L1 in human brain tissue with SIPA1L1 antibody at 20 μg/mL.
Western blot analysis of SIPA1L1 in rat brain tissue lysate with SIPA1L1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SIPA1L1 Antibody

CATALOG NUMBER: 5491

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIPA1L1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SIPA1L1.
The immunogen is located within amino acids 40 - 90 of SIPA1L1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SIPA1L1 antibody can be used for detection of SIPA1L1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SIPA1L1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIPA1L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIPA1L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIPA1L1
Additional Names:
SIPA1L1 Antibody: E6TP1, E6TP1, KIAA0440, Signal-induced proliferation-associated 1-like protein 1, High-risk human papilloma viruses E6 oncoproteins targeted protein 1, SIPA1-like protein 1
Protein Accession Number:
NP_056371
Protein Gi Number:
7662126
Ncbi Gene Id Number:
26037
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIPA1L1 Antibody: Signal-induced proliferation associated-like protein 1 (SIPA1L1) is a member of the SIPA1 family of RapGAPs. SIPA1L1 was initially identified as a binding partner and degradation target of the E6 oncoprotein of high-risk papillomaviruses. Recently, it was discovered that Casein kinase I epsilon (CKIe), a Wnt-regulated kinase that regulates Wnt/b-catenin signaling, also can bind to the carboxy-terminus of SIPA1L1. CKIe phosphorylates SIPA1L1, thereby reducing its stability and alleviating its inhibition of Rap1, a protein required for Wnt8/CKIe-mediated gastrulation during embryogenesis, suggesting SIPA1L1 plays important roles in embryo development as well as control of cell proliferation.
Background Reference 1:
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
Background Reference 2:
Gao Q, Srinivasan S, Boyer SN, et al. The E6 oncoproteins of high-risk papillomaviruses bind to a novel putative GAP protein, E6TP1, and target it for degradation. Mol. Cell Biol.1999; 19:733-44.
Background Reference 3:
Tsai I-C, Amack JD, Gao Z-H, et al. A Wnt-CKI e-Rap1 pathway regulates gastrulation by modulating SIPA1L1, a Rap GTPase activating protein. Dev. Cell2007; 12:335-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5491

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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