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Western blot analysis of SIPA1L2 in rat brain tissue lysate with SIPA1L2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SIPA1L2 in human brain tissue with SIPA1L2 antibody at 5 μg/mL.
Immunofluorescence of SIPA1L2 in human brain tissue with SIPA1L2 antibody at 20 μg/mL.
Western blot analysis of SIPA1L2 in rat brain tissue lysate with SIPA1L2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SIPA1L2 Antibody

CATALOG NUMBER: 5493

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIPA1L2 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human SIPA1L2.
The immunogen is located within the first 50 amino acids of SIPA1L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SIPA1L2 antibody can be used for detection of SIPA1L2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SIPA1L2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIPA1L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIPA1L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIPA1L2
Additional Names:
SIPA1L2 Antibody: SPAL2, KIAA1389, Signal-induced proliferation-associated 1-like protein 2, SIPA1-like protein 2
Protein Accession Number:
NP_065859
Protein Gi Number:
112421013
Ncbi Gene Id Number:
57568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIPA1L2 Antibody: Signal-induced proliferation associated-like protein 2 (SIPA1L2) is a member of the SIPA1 family of RapGAPs. Little is known of the role of the SIPA1L2 protein, but recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is likely that SIPA1L2 plays a role in embryo development and cell proliferation, possibly including oncogenesis.
Background Reference 1:
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
Background Reference 2:
Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell2003; 4:55-65.
Background Reference 3:
Park YG, Zhao X, Lesueur F, et al. Sipa1 is a candidate for underlying the metastasis efficiency modifier locus, Mtes. Nat. Genet.2005; 37:1055-62.
Background Reference 4:
Tsai I-C, Amack JD, Gao Z-H, et al. A Wnt-CKI e-Rap1 pathway regulates gastrulation by modulating SIPA1L2, a Rap GTPase activating protein. Dev. Cell2007; 12:335-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5493

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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