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Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.
Immunohistochemistry of SIPA1L3 in human brain tissue with SIPA1L3 antibody at 5 μg/mL.
Immunofluorescence of SIPA1L3 in Human Brain cells with SIPA1L3 antibody at 20 μg/mL.
Western blot analysis of SIPA1L3 in SK-N-SH lysate with SIPA1L3 antibody at 1 μg/mL.
Trial Size

SIPA1L3 Antibody

CATALOG NUMBER: 5495

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIPA1L3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SIPA1L3.
The immunogen is located within amino acids 80 - 130 of SIPA1L3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SIPA1L3 antibody can be used for detection of SIPA1L3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SIPA1L3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIPA1L3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIPA1L3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIPA1L3
Additional Names:
SIPA1L3 Antibody: SPAL3, KIAA0545, SPAL3, Signal-induced proliferation-associated 1-like protein 3, SPA-1-like protein 3, SIPA1-like protein 3
Protein Accession Number:
NP_055888
Protein Gi Number:
71143119
Ncbi Gene Id Number:
23094
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIPA1L3 Antibody: Signal-induced proliferation associated-like protein 2 (SIPA1L3) is a member of the SIPA1 family of RapGAPs. SIPA1L3 was cloned as a novel molecular component of tight junctions and adherens junctions, suggesting that may function to balance Rap1 by counteracting RapGEFs. Recent studies of SIPA indicate that its deregulation can cause myeloproliferative stem cell disorders in mice and increased metastases in human cancers. Other studies suggest SIPA1L1 may play important roles in embryo development and control of cell proliferation. Based on the amount of homology between SIPA family members, it is possible that SIPA1L3 plays other roles development and cell proliferation.
Background Reference 1:
Minato N and Hattori M. SPA-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci.2009; 100:17-23.
Background Reference 2:
Matsuda M, Kobayashi Y, Masuda S, et al. Identification of adherences junction-associated GTPase activating proteins by the fluorescence localization-based expression cloning. Exp. Cell Res.2008; 314:939-49.
Background Reference 3:
Ishida D, Kometani K, Yang H, et al. Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell2003; 4:55-65.
Background Reference 4:
Park YG, Zhao X, Lesueur F, et al. Sipa1 is a candidate for underlying the metastasis efficiency modifier locus, Mtes. Nat. Genet.2005; 37:1055-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5495

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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