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Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 μg/mL.
Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 μg/mL.
Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 μg/mL.
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SIRP alpha Antibody

CATALOG NUMBER: 1125

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1.
The immunogen is located within the last 50 amino acids of SIRP alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
SIRP alpha antibody can be used for Western blot at 0.5 - 1 μg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
Recognizes SIRP alpha 1, 2 and 3.
Positive Control 1:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2:
Cat. No. 17-008 - THP-1 Cell Slide
Predicted Molecular Weight:
75 - 110 kDa
Purification:
SIRP alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
SIRP alpha Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRP antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRPA
Additional Names:
SIRP alpha Antibody: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, BIT, MYD1, Tyrosine-protein phosphatase non-receptor type substrate 1, Brain Ig-like molecule with tyrosine-based activation motifs, SHP substrate 1
Protein Accession Number:
NP_542970
Protein Gi Number:
18426911
Ncbi Gene Id Number:
140885
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Background Reference 1:
Kharitonenkov A, et al. Nature 1997;386:181-186.
Background Reference 2:
Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899
Background Reference 3:
Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67
Background Reference 4:
Brooke GP, et al. Eur J Immunol 1998;28:1-11

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1125

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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