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Antibody used in WB on Human Thymus at 0.5 ug/ml.
Antibody used in WB on Human Thymus at 0.5 ug/ml.

SIRT1 Antibody

CATALOG NUMBER: 27-577

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIRT1 antibody can be used for detection of SIRT1 by ELISA at 1:62500. SIRT1 antibody can be used for detection of SIRT1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Predicted Molecular Weight:
82 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SIRT1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SIRT1
Additional Names:
SIRT1, SIR2L1
Protein Accession Number:
NP_036370
Protein Gi Number:
7657575
Ncbi Gene Id Number:
23411
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.
Background Reference 1:
Solomon, J.M., et al., (2006) Mol. Cell. Biol. 26 (1), 28-38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-577

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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