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43-119 (1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
43-119 (1µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

SIRT1 Antibody

CATALOG NUMBER: 43-119

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EITEKPPRTQKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 80.3kDa according to Mouse NP_062786.1). Preliminary testing also showed a band at approx 120xkDa in Fetal Mouse Brain lysate. The observed molecular weights correspond to different antibodies in the literature and from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Predicted Molecular Weight:
Approx 120kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SIRT1
Additional Names:
SIRT1 , sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , RP11-57G10.3, SIR2L1 , OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
Protein Accession Number:
NP_036370.2
Protein Gi Number:
7657575
Ncbi Gene Id Number:
23411
Background Reference 1:
Chen D, Steele AD, Lindquist S, Guarente L. Increase in activity during calorie restriction requires Sirt1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-119

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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