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Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 μg/mL.
Immunofluorescence of SIRT1 in rat lung tissue with SIRT1 antibody at 20 μg/mL.
Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 μg/mL.
Trial Size

SIRT1 Antibody

CATALOG NUMBER: 5765

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIRT1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SIRT1.
The immunogen is located within the first 50 amino acids of SIRT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SIRT1 antibody can be used for detection of SIRT1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
SIRT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SIRT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRT1
Additional Names:
SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1
Protein Accession Number:
NP_036370
Protein Gi Number:
7657575
Ncbi Gene Id Number:
23411
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT1 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
Background Reference 1:
Salminen A. SIRT1: regulation of longevity via autophagy.
Background Reference 2:
Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8.
Background Reference 3:
Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
Background Reference 4:
Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5765

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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