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Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Antibody used in WB on Mouse pancreas at: 1:1000 (Lanes: 1. 30 ug mouse pancreas extract 2. 30 ug mouse pancreas extract 3. 30 ug mouse pancreas extract).
Antibody used in WB on Human Brain at 0.2-1 ug/ml.

SIRT2 Antibody

CATALOG NUMBER: 27-575

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIRT2 antibody can be used for detection of SIRT2 by ELISA at 1:312500. SIRT2 antibody can be used for detection of SIRT2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Predicted Molecular Weight:
40 kDa, 43 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SIRT2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SIRT2
Additional Names:
SIRT2, SIR2, SIR2L, SIR2L2
Protein Accession Number:
NP_085096
Protein Gi Number:
13775602
Ncbi Gene Id Number:
22933
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT2 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT2 is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
Background Reference 1:
Borra, M.T., et al., (2004) Biochemistry 43 (30), 9877-9887.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-575

List Size:
100 ul

List Price:

$519.00

Datasheet
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