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Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 μg/mL.
Immunohistochemical staining of human brain tissue using Sirt2 antibody at 2.5 μg/mL.
Immunofluorescence of sirt2 in human brain tissue with sirt2 antibody at 20 μg/mL.
Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 μg/mL.
Trial Size

SIRT2 Antibody

CATALOG NUMBER: 4487

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIRT2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2.
The immunogen is located within the first 50 amino acids of SIRT2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SIRT2 antibody can be used for detection of SIRT2 by Western blot at 2.5 - 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SIRT2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIRT2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRT2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRT2
Additional Names:
SIRT2 Antibody: SIR2, SIR2L, SIR2L2, NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2
Protein Accession Number:
NP_036369
Protein Gi Number:
13775600
Ncbi Gene Id Number:
22933
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
Background Reference 1:
Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol.2007; 21:1745-55.
Background Reference 2:
Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: SIR2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. Biochem. Biophys. Res. Commun.1999; 260:273-279.
Background Reference 3:
North BJ, Marshall BL, Borra MT, et. al. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol. Cell.2003; 11:437-44.
Background Reference 4:
Inoue T, Hiratsuka M, Osaki M, et al. The molecular biology of mammalian SIRT proteins: SIRT2 in cell cycle regulation. Cell Cycle2007; 6:1011-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4487

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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