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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Antibody used in IHC on Human kidney.

SIRT3 Antibody

CATALOG NUMBER: 27-579

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SIRT3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SIRT3 antibody can be used for detection of SIRT3 by ELISA at 1:62500. SIRT3 antibody can be used for detection of SIRT3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
28 kDa, 44 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SIRT3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SIRT3
Additional Names:
SIRT3, SIR2L3
Protein Accession Number:
NP_001017524
Protein Gi Number:
63054862
Ncbi Gene Id Number:
23410
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The SIRT3 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT3 encodes a protein that is included in class I of the sirtuin family.
Background Reference 1:
Onyango, P., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 15 99(21), 13653-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-579

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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