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Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SIRT3 Antibody

CATALOG NUMBER: 5767

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SIRT3 antibody was raised against a 17 amino acid synthetic peptide near the center of human SIRT3.
The immunogen is located within amino acids 180 - 230 of SIRT3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIRT3 antibody can be used for detection of SIRT3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
SIRT3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SIRT3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRT3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRT3
Additional Names:
SIRT3 Antibody: SIR2L3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, hSIRT3
Protein Accession Number:
NP_036371
Protein Gi Number:
6912660
Ncbi Gene Id Number:
23410
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT3 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence, lifespan extension and several age-related diseases. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death.
Background Reference 1:
Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
Background Reference 2:
Lavu S, Boss O, Elliott PJ, et al. Sirtuins - novel therapeutic targets to treat age-associated diseases. Nat. Rev. Drug. Disc.2008; 7:841-53.
Background Reference 3:
Onyango P, Celic I, McCaffery JM, et al. SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria. Proc. Natl. Acad. Sci. USA.2002; 99:13653-8.
Background Reference 4:
Hirschey MD, Shimazu T, Goetzman E, et al. SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature2010; 464:121-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5767

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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