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Western blot analysis of SIRT4 in (A) human and (B) mouse liver tissue lysate with SIRT4 antibody at 0.5 μg/mL
Western blot analysis of SIRT4 in (A) human and (B) mouse liver tissue lysate with SIRT4 antibody at 0.5 μg/mL
Trial Size

SIRT4 Antibody

CATALOG NUMBER: 5769

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SIRT4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human SIRT4.
The immunogen is located within amino acids 80 - 130 of SIRT4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIRT4 antibody can be used for detection of SIRT4 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 37 kDa
Observed: 40 kDa
Purification:
SIRT4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SIRT4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRT4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRT4
Additional Names:
SIRT4 Antibody: SIR2L4, SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4
Protein Accession Number:
EAW98182
Protein Gi Number:
119618588
Ncbi Gene Id Number:
23409
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT4 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT4 localizes to mitochondria, inhibits glutamate dehydrogenase, and is thought to be involved in the regulation of insulin secretion.
Background Reference 1:
Salminen A. SIRT1: regulation of longevity via autophagy. Cell Signal2009; 21:1356-60.
Background Reference 2:
Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8.
Background Reference 3:
Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
Background Reference 4:
Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5769

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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