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Western blot analysis of SIRT5 in A431 cell lysate with SIRT5 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of SIRT5 in A431 cell lysate with SIRT5 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

SIRT5 Antibody

CATALOG NUMBER: 5771

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Bovine: (88%), Chicken: (82%)
Immunogen:
SIRT5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SIRT5.
The immunogen is located within the last 50 amino acids of SIRT5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SIRT5 antibody can be used for detection of SIRT5 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
SIRT5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SIRT5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIRT5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIRT5
Additional Names:
SIRT5 Antibody: SIR2L5, SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5
Protein Accession Number:
NP_036373
Protein Gi Number:
6912664
Ncbi Gene Id Number:
23408
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIRT5 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT5 localizes to mitochondria, deacetylates carbamoyl phosphate synthetase 1, and is involved in the regulation of the urea cycle.
Background Reference 1:
Salminen A. SIRT1: regulation of longevity via autophagy. Cell Signal2009; 21:1356-60.
Background Reference 2:
Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8.
Background Reference 3:
Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
Background Reference 4:
Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5771

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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