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Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SKA3 Antibody

CATALOG NUMBER: 5405

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3.
The immunogen is located within amino acids 290 - 340 of SKA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
SKA3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SKA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SKA3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SKA3
Additional Names:
SKA3 Antibody: RAMA1, C13orf3, RAMA1, Spindle and kinetochore-associated protein 3
Protein Accession Number:
NP_659498
Protein Gi Number:
260763910
Ncbi Gene Id Number:
221150
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SKA3 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
Background Reference 1:
Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.
Background Reference 2:
Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.
Background Reference 3:
Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.
Background Reference 4:
Daum JR, Wren JD, Daniel JJ, et al. Ska3 is required for spindle checkpoint silencing and maintenance of chromosome cohesion in mitosis. Curr. Biol.2009; 19:1467-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5405

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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