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Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μg/ml.
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μg/ml.
Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.
Trial Size

SKI2W Antibody

CATALOG NUMBER: 7993

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.
The immunogen is located within amino acids 60 - 110 of SKI2W.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1415 - Mouse Stomach Tissue Lysate
Positive Control 2:
Cat. No. 10-801 - Human Stomach Tissue Slide
Predicted Molecular Weight:
Predicted: 113, 137 kDa
Observed: 135 kDa
Purification:
SKI2W antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SKI2W antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SKI2W antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SKIV2L
Additional Names:
Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L
Protein Accession Number:
NP_008860
Protein Gi Number:
67782311
Ncbi Gene Id Number:
6499
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1,2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3,4).
Background Reference 1:
Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6.
Background Reference 2:
Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77.
Background Reference 3:
Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47.
Background Reference 4:
Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7993

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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