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Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.
Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.
Trial Size

SkiP Antibody

CATALOG NUMBER: 2261

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP.
The immunogen is located within the last 50 amino acids of SkiP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
SkiP antibody can be used for detection of SkiP by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Positive Control 2:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 3:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 58, 62 kDa
Observed: 65 kDa 
Purification:
SkiP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SkiP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
SkiP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNW1
Additional Names:
SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP
Protein Accession Number:
NP_036377
Protein Gi Number:
6912676
Ncbi Gene Id Number:
22938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.
Background Reference 1:
Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
Background Reference 2:
Li Y, Turck CM, Teumer JK, et al. Unique sequence, SkiP, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
Background Reference 3:
Luo K. SkiP and SkiP: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
Background Reference 4:
Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2261

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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