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Western blot analysis of SKIV2L2 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SKIV2L2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SKIV2L2 Antibody in MCF-7 cell line lysates (35ug/lane)

SKIV2L2 Antibody

CATALOG NUMBER: 63-621

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SKIV2L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-263 amino acids from the Central region of human SKIV2L2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
118 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SKIV2L2
Additional Names:
Superkiller viralicidic activity 2-like 2, ATP-dependent RNA helicase SKIV2L2, TRAMP-like complex helicase, SKIV2L2, KIAA0052, Mtr4
Protein Accession Number:
P42285
Protein Gi Number:
71153172
Ncbi Gene Id Number:
23517
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SKIV2L2 may be involved in pre-mRNA splicing.
Background Reference 1:
Choudhary C.,et.al., Science 325:834-840(2009).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-621

List Size:
400 ul

List Price:

$495.00

Datasheet
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