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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

SLC12A1 Antibody

CATALOG NUMBER: 29-559

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SLC12A1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
SLC12A1 antibody can be used for detection of SLC12A1 by ELISA at 1:312500. SLC12A1 antibody can be used for detection of SLC12A1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa, 121 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store SLC12A1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
SLC12A1
Additional Names:
SLC12A1, BSC1, MGC48843, NKCC2
Protein Accession Number:
AAH40138
Protein Gi Number:
25304083
Ncbi Gene Id Number:
6557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-559

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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