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42-498 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
42-498 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle and membrane staining. The nuc
42-498 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
42-498 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

SLC12A2 Antibody

CATALOG NUMBER: 42-498

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NALQHSIRLSGVEDH
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC12A2
Additional Names:
SLC12A2, solute carrier family 12 (sodium/potassium/chloride transporters), member 2, BSC, BSC2, MGC104233, NKCC1, basolateral Na-K-Cl symporter, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
Protein Accession Number:
NP_001037.1,NP_001243390.1
Protein Gi Number:
4506975
Ncbi Gene Id Number:
6558

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-498

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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