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Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 5 μg/ml.
Immunofluorescence of SLC1A7 in mouse kidney tissue with SLC1A7 antibody at 20 μg/ml.
Western blot analysis of SLC1A7 in C2C12 cell lysate with SLC1A7 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SLC1A7 Antibody

CATALOG NUMBER: 8083

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7.
The immunogen is located within amino acids 460 - 510 of SLC1A7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.
Positive Control 1:
Cat. No. 1285 - C2C12 Cell Lysate
Predicted Molecular Weight:
Predicted: 17, 52, 62, 68 kDa
Observed: 52 kDa
Purification:
SLC1A7 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC1A7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SLC1A7
Additional Names:
Solute carrier family 1 (glutamate transporter) member 7, AAAT, excitatory amino acid transporter 5, EAAT5
Protein Accession Number:
NP_001274524
Protein Gi Number:
567316146
Ncbi Gene Id Number:
6512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
Background Reference 1:
Arriza JL, Eliasof S, Kavanaugh MP, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 1997; 94:4155-60.
Background Reference 2:
Lee A, Anderson AR, Stevens M, et al. Excitatory amino acid transporter 5 is widely expressed in peripheral tissues. Eur. J. Histochem. 2013; 57:e11.
Background Reference 3:
Schneider N, Cordeiro S, Machtens JP, et al. Functional properties of the retinal glutamate transporters GLT-1c and EAAT5. J. Biol. Chem. 2014; 289:1815-24.
Background Reference 4:
Boehmer C, Rajamanickam J, Schniepp R, et al. Regulation of the excitatory amino acid transporter EAAT5 by the serum and glucocorticoid dependent kinases SGK1 and SGK3. Biochem. Biophys. Res. Commun. 2005; 329:738-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8083

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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