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Western blot analysis of extracts of various cell lines, using SLC25A5 antibody (16-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using SLC25A5 antibody (16-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SLC25A5 antibody (16-156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

SLC25A5 Antibody

CATALOG NUMBER: 16-156

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SLC25A5 (NP_001143.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
LO2
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse heart
Positive Control 5:
Mouse kidney
Positive Control 6:
Rat kidney
Predicted Molecular Weight:
Observed: 33kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC25A5
Additional Names:
2F1, AAC2, ANT2, T2, T3, ADP/ATP translocase 2, ADP,ATP carrier protein, fibroblast isoform, adenine nucleotide translocator 2 (fibroblast), solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Ncbi Gene Id Number:
292
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-156

List Size:
100 uL

List Price:

$436.00

Datasheet
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