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Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SLC29A1 in human ovary tissue with SLC29A1 antibody at 5 μg/mL.
Immunofluorescence of SLC29A1 in human ovary tissue with SLC29A1 antibody at 20 μg/mL.
Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

SLC29A1 Antibody

CATALOG NUMBER: 8125

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%), Mouse: (75%)
Immunogen:
SLC29A1 antibody was raised against a 17 amino acid peptide near the center of human SLC29A1.
The immunogen is located within amino acids 240 - 290 of SLC29A1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SLC29A1 antibody can be used for detection of SLC29A1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SLC29A1 antibody is human specific. SLC29A1 antibody is predicted to not cross-react with other SLC29 proteins.
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Positive Control 2:
Cat. No. 11-201 - Human Ovary Tissue Slide
Predicted Molecular Weight:
Predicted: 50 kDa
Observed: 53 kDa
Purification:
SLC29A1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC29A1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC29A1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SLC29A1
Additional Names:
Solute carrier family 29 member 1, Equilibrative nucleoside transporter 1, ENT1
Protein Accession Number:
NP_004946
Protein Gi Number:
4826716
Ncbi Gene Id Number:
2030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SLC29A1 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A1 is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium (3). As a nucleoside transporter, SLC29A1 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4).
Background Reference 1:
Griffiths M, Beaumont N, Yao SY, et al. Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat. Med. 1997; 3:89-93.
Background Reference 2:
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Background Reference 3:
Mangravite LM, Xiao G, and Giacomini KM. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am. J. Physiol. Renal Physiol. 284:F902-10.
Background Reference 4:
Zimmerman EI, Huang M, Leisewitz AV, et al. Identification of a novel point mutation in ENT1 that confers resistance to Ara-C in human T cell leukemia CCRF-CEM cells. FEBS Lett. 2009; 583:425-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8125

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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