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Western blot analysis of SLC29A2 in human bladder tissue lysate with SLC29A2 antibody at 1 μg/ml.
Immunohistochemistry of SLC29A2 in human bladder tissue with SLC29A2 antibody at 2.5 μg/mL.
Immunofluorescence of SLC29A2 in human bladder tissue with SLC29A2 antibody at 20 μg/ml.
Western blot analysis of SLC29A2 in human bladder tissue lysate with SLC29A2 antibody at 1 μg/ml.
Trial Size

SLC29A2 Antibody

CATALOG NUMBER: 8127

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SLC29A2 antibody was raised against a 19 amino acid peptide near the center of human SLC29A2.
The immunogen is located within amino acids 250 - 300 of SLC29A2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SLC29A2 antibody can be used for detection of SLC29A2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SLC29A2 antibody is human specific. SLC29A2 antibody is predicted to not cross-react with other SLC29 proteins.
Positive Control 1:
Cat. No. 1310 - Human Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 50 kDa
Observed: 50 kDa
Purification:
SLC29A2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC29A2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC29A2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SLC29A2
Additional Names:
Solute carrier family 29 member 2, Equilibrative nucleoside transporter 2, ENT2, DER12, HNP36
Protein Accession Number:
NP_001523
Protein Gi Number:
38708299
Ncbi Gene Id Number:
3177
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SLC29A2 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A2 is a transmembrane glycoprotein that mediates the cellular uptake of nucleosides from the surrounding medium (3). As a nucleoside transporter, SLC29A2 plays an important role in the uptake of nucleoside-based anti-cancer drugs; polymorphisms of point mutations in the gene encoding this protein may affect the efficacy of these drugs (4).
Background Reference 1:
Williams JB and Lanahan AA. A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protein. Biochim. Biophys. Res. Commun. 1995; 213:325-33.
Background Reference 2:
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Background Reference 3:
Mangravite LM, Xiao G, and Giacomini KM. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am. J. Physiol. Renal Physiol. 284:F902-10.
Background Reference 4:
Owen RP, Lagpacan LL, Taylor TR, et al. Functional characterization and haplotype analysis of polymorphisms in the human equilabrative nucleoside transporter, ENT2. Drug Metab. Dispos. 2006; 34:12-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8127

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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