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Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SLC29A3 in rat colon tissue with SLC29A3 antibody at 5 μg/mL.
Immunofluorescence of SLC29A3 in rat colon muscle tissue with SLC29A3 antibody at 20 μg/mL.
Western blot analysis of SLC29A3 in mouse bladder tissue lysate with SLC29A3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

SLC29A3 Antibody

CATALOG NUMBER: 8129

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
SLC29A3 antibody was raised against a 19 amino acid peptide near the amino terminus of human SLC29A3.
The immunogen is located within the first 50 amino acids of SLC29A3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SLC29A3 antibody can be used for detection of SLC29A3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
SLC29A3 antibody is human, mouse and rat reactive. At least two isoforms of SLC29A3 are known to exist; this antibody will detect both isoforms. SLC29A3 antibody is predicted to not cross-react with other SLC29 proteins.
Positive Control 1:
Cat. No. 1410 - Mouse Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 52 kDa
Observed: 51 kDa
Purification:
SLC29A3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC29A3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC29A3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SLC29A3
Additional Names:
Solute carrier family 29 member 3, Equilibrative nucleoside transporter 3, ENT3, HCLAP, HJCD, PHID
Protein Accession Number:
NP_060814
Protein Gi Number:
148596922
Ncbi Gene Id Number:
55315
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SLC29A3 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A3 is a transmembrane glycoprotein that localizes to the lysosomal membrane and is a broad selectivity, low affinity nucleoside transporter (3). Mutations in the SLC29A3 gene have been associated with H syndrome, which is characterized by cutaneous hyperpigmentation and hypertrichosis, hepatosplenomegaly, heart anomalies, and hypogonadism (4). A related disorder, PHID (pigmented hypertrichosis with insulin-dependent diabetes mellitus), has also been associated with mutations at this locus (5).
Background Reference 1:
Hyde RJ, Cass CE, Young JD, et al. The ENT family of eukaryotic nucleoside and nucleobase transporters: recent advances in the investigation of structure/function relationships and the identification of novel isoforms. Mol. Membr. Biol. 2001; 18:53-63.
Background Reference 2:
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Background Reference 3:
Baldwin SA, Yao SY, Hyde RJ, et al. Functional characterization of novel human and mouse equilibrative nucleoside transporters (hENT3 and mENT3) located in intracellular membranes. J. Biol. Chem. 2005; 280:15880-7.
Background Reference 4:
Priya TP, Philip N, Molho-Pessach V, et al. H syndrome: novel and recurrent mutations in SLC29A3. Br. J. Dermatol. 2010; 162:1132-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8129

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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