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42-800 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-800 (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-800 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

SLC2A4 Antibody

CATALOG NUMBER: 42-800

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow, Pig
Immunogen:
The immunogen for this antibody is: C-TELEYLGPDEND
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997Mar 14;272(11):7085-92.; PMID: 9054401). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SLC2A4
Additional Names:
SLC2A4, solute carrier family 2 (facilitated glucose transporter), member 4, GLUT4, glucose transporter 4, insulin-responsive glucose transporter type 4
Protein Accession Number:
NP_001033.1
Protein Gi Number:
4507011
Ncbi Gene Id Number:
6517

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-800

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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