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Western blot analysis of extracts of various cell lines, using SLC32A1 antibody (18-955) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using SLC32A1 antibody (18-955) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SLC32A1 antibody (18-955) (40x lens).
Immunofluorescence analysis of Y79 cells using SLC32A1 Polyclonal Antibody (18-955) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SLC32A1 antibody (18-955) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

SLC32A1 Antibody

CATALOG NUMBER: 18-955

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human SLC32A1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse spinal cord
Positive Control 2:
Mouse brain
Predicted Molecular Weight:
Observed: 53kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC32A1
Additional Names:
VGAT, VIAAT, vesicular inhibitory amino acid transporter, GABA and glycine transporter, hVIAAT, solute carrier family 32 (GABA vesicular transporter), member 1, vesicular GABA transporter
Ncbi Gene Id Number:
140679
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-955

List Size:
100 uL

List Price:

$436.00

Datasheet
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