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Western blot analysis of SLC35D3 in HeLa cell lysate with SLC35D3 antibody at 1 μg/mL.
Immunocytochemistry of SLC35D3 in HeLa cells with SLC35D3 antibody at 2.5 μg/mL.
Immunofluorescence of SLC35D3 in HeLa cells with SLC35D3 antibody at 5 μg/mL.
Western blot analysis of SLC35D3 in HeLa cell lysate with SLC35D3 antibody at 1 μg/mL.
Trial Size

SLC35D3 Antibody

CATALOG NUMBER: 6547

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
SLC35D3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SLC35D3.
The immunogen is located within the last 50 amino acids of SLC35D3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SLC35D3 antibody can be used for detection of SLC35D3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of SLC35D3 are known to exist; this antibody will recognize both isoforms. SLC35D3 antibody is predicted to not cross-react with SLC35D1 or SLC35D2.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 45 kDa
Purification:
SLC35D3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC35D3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC35D3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SLC35D3
Additional Names:
SLC35D3 Antibody: FRCL1, bA55K22.3, FRCL1, Solute carrier family 35 member D3, Fringe connection-like protein 1
Protein Accession Number:
AAH87842
Protein Gi Number:
56912198
Ncbi Gene Id Number:
340146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SLC35D3 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The novel protein SLC35D3 is highly homologous to SLC35D1 and SLC35D2, both of which transport UDP-glucuronic acid and UDP-N-acetylgalactosamine, suggesting that SLC35D3 is also involved in the transport of nucleotide sugars. It has been suggested that SLC35D3 regulates platelet dense granules, lysosome-related organelles which contain high concentrations of several biologically important low molecular weight molecules necessary for normal blood homeostasis.
Background Reference 1:
Ishida N and Kawakita M. Molecular physiology and pathology of the nucleotide sugar transporter family (SLC35). Pflugers Arch. 2004; 447:768-75.
Background Reference 2:
Suda T, Kamiyama S, Suzuki M, et al. Molecular cloning and characterization of a human multisubstrate specific nucleotide-sugar transporter homologous to Drosophila fringe connection. J. Biol. Chem. 2004; 279:26469-74.
Background Reference 3:
Chintala S, Tan J, Gautam R, et al. The Slc35d3 gene, encoding an orphan nucleotide sugar transporter, regulates platelet-dense granules. Blood 2007; 109:1533-40

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6547

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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