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Western blot analysis of extracts of various cell lines, using SLC39A4 antibody (19-072) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using SLC39A4 antibody (19-072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SLC39A4 antibody (19-072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SLC39A4 antibody (19-072) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SLC39A4 antibody (19-072) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

SLC39A4 Antibody

CATALOG NUMBER: 19-072

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-327 of human SLC39A4 (NP_570901.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
293T
Positive Control 3:
Mouse liver
Predicted Molecular Weight:
Observed: 75kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC39A4
Additional Names:
SLC39A4, AEZ, FLJ20327, MGC74741, ZIP4, AWMS2
Ncbi Gene Id Number:
55630
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-072

List Size:
100 uL

List Price:

$436.00

Datasheet
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